Monday 06th of September 2010
DNA Enzymes
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 ThermoPhage® Polynucleotide Kinase
ThermoPhage™ Polynucleotide Kinase is a novel thermoactive kinase catalysing 5’ phosphorylation of DNA at elevated temperatures and is the only available thermostable enzyme of this kind. The enzyme has very low 3' phosphatase activity. Thermophage™ Polynucleotide Kinase is well suited for various applications including radioactive labelling of nucleic acids. Available as 500 units and 1000 units together with 10x reaction buffers
For further information read more below or download the product sheet (pdf format). See also our file archive. |
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 Ice and Fire™ Phosphorylation Kit
The Ice and Fire™ Phosphorylation kit includes optimized reagents for the 5’ end labeling of nucleic acids. The novel protocol provides easy "one-step" handling by combining the low temperature activity of thermolabile Shrimp Alkaline Phosphatase (SAP) and the high temperature activity of the thermostable ThermoPhage™ polynucleotide kinase (PNK). Available as kit for 50 reactions |
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 Thermus scotoductus DNA ligase
Tsc DNA ligase from Thermus scotoductus is a thermostable NAD+ dependent double-stranded DNA ligase with activity at temperatures between 15 and 75°C. The enzyme has no activity on blunt-end DNA fragments. Available as 500 units and 2000 units together with 10x reaction buffers.
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 Rhodothermus marinus DNA ligase
Rma DNA ligase from Rhodothermus marinus is a thermostable NAD+ dependent double-stranded DNA ligase with broad temperatures range between 5 and 65°C. The enzyme catalyzes ligation of DNA with high fidelity and is suitable for various applications including gene synthesis. Available as 500 units and 2000 units together with 10x reaction buffers.
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